This book provides a comprehensive list of the methods used in basic and applied geminivirus research. Chapters guide readers through foundational methods, advanced techniques in cloning, sequencing, phylogenetic, recombination analysis, diagnostics, and virus-host interaction studies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Geminiviruses: Methods and Protocols aims to be a practical laboratory guide and serve as a valuable "one-stop-shop" for researchers new to geminiviruses, facilitating the establishment of a robust geminivirus research program.