This second volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. Chapters describe the isolation, culture of diverse skin cells, skin models, and managing these cells within co-culture systems. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions tothe material, lists of necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and a Notes section which highlightstips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Skin Tissue Engineering*: Methods and Protocols, Second Edition* aims to be comprehensive guide forresearchers in the field.