This updated collection features protocols that reflect the continued expansion of stem cell niche research. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the second edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Niche: Methods and Protocols, Third Edition serves as an ideal guide for both experts and novices in the stem cell field.